Structure of the carboxypeptidase t from Thermoactinomyces vulgaris complex with L - phenyl lactate
- Авторлар: Akparov V.K.1, Konstantinova G.E.1, Timofeev V.I.1, Shvetsov M.B.2, Kuranova I.P.1,2
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Мекемелер:
- Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”
- Moscow Institute of Physics and Technology
- Шығарылым: Том 69, № 3 (2024)
- Беттер: 422-428
- Бөлім: STRUCTURE OF MACROMOLECULAR COMPOUNDS
- URL: https://jdigitaldiagnostics.com/0023-4761/article/view/673174
- DOI: https://doi.org/10.31857/S0023476124030067
- EDN: https://elibrary.ru/XOPTJS
- ID: 673174
Дәйексөз келтіру
Аннотация
The crystal structure of the metallocarboxypeptidase T from Thermoactinomyces vulgaris complex with L-phenylactate was obtained with a resolution of 1.73 Å. Unlike pancreatic carboxypeptidase A, which binds one L-phenylactate molecule, in complex with CPT, the ligand occupies both S1 and S1ʹ sites of the active center simultaneously. In this case, conformational changes occur that differ from the changes caused by the alternate occupation of the S1 and S1ʹ sites by BOC-leucine and benzylsuccinic acid. These changes concern the residues E277, E59, L254, G192, S127 and Y218 and reach a span of 0.77 Å. A conclusion is made about the possible role of these residues in the recognition and catalysis of substrates by carboxypeptidase T.
Толық мәтін

Авторлар туралы
V. Akparov
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”
Хат алмасуға жауапты Автор.
Email: valery.akparov@yandex.ru
Ресей, Moscow
G. Konstantinova
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”
Email: valery.akparov@yandex.ru
Ресей, Moscow
V. Timofeev
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”
Email: valery.akparov@yandex.ru
Ресей, Moscow
M. Shvetsov
Moscow Institute of Physics and Technology
Email: valery.akparov@yandex.ru
Ресей, Dolgoprudny
I. Kuranova
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”; Moscow Institute of Physics and Technology
Email: valery.akparov@yandex.ru
Ресей, Moscow; Dolgoprudny
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