Integrated approach to early detection of cotton disease resistance
- Autores: Akhmedzhanov I.G.1, Khotamov M.M.2
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Afiliações:
- Institute of Biophysics and Biochemistry at the National University of Uzbekistan
- Institute of Genetics and Plant Experimental Biology of the Academy of Sciences of the Republic of Uzbekistan
- Edição: Volume 59, Nº 5 (2025)
- Páginas: 408-415
- Seção: PHYTOPATHOGENIC FUNGI
- URL: https://jdigitaldiagnostics.com/0026-3648/article/view/691639
- DOI: https://doi.org/10.31857/S0026364825050064
- EDN: https://elibrary.ru/btawrp
- ID: 691639
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Resumo
The functional features of the implementation of cotton protective reactions to the most dangerous pathogens, Verticillium dahliae and Fusarium oxysporum, Xanthomonas malvacearum and Rhizoctonia solani – were studied. The hypersensitivity reaction of cotton tissues infected with pathogens was controlled by methods of observing the movement and size of the zone of fluorescent substances and determining the amount of toxic compounds for pathogens – phytoalexins. Infection of cotton with Verticillium dahliae and Fusarium oxysporum already in the first days of incubation led to a bright blue fluorescence that spread upwards towards the growth point of the experimental plants. In cotton infected with Xanthomonas malvacearum and Rhizoctonia solani the color of the fluorescent zones was less intense. The rate of spread through plant tissues, especially at the initial stages of the latent period, was significantly lower. In addition, the content of post-infection inhibitors in the tissues of xylem vessels of cotton infected with gummosis and root rot was recorded at a significantly lower level compared to the experimental plants infected with Fusarium and Verticillium wilt. On the 20th day of incubation, a significant increase in the total content of phytoalexins was noted in experimental cotton plants infected with root rot and gummosis, but the amount of the main phytoalexin – isohemigossypol in the tissues of these plants reached only 50% of the level of samples infected with Fusarium and Verticillium wilt. A comparative analysis of the effect of the studied pathogens on the intensity of the hypersensitivity reaction and the parameters of chlorophyll fluorescence induction indicate the possibility of using these methods at the initial stages of the incubation period in infected plant tissues for early detection of cotton disease resistance. The establishment of a positive correlation between the results of fluorescent analysis and the indicators of the effectiveness of the hypersensitivity reaction indicate the advisability of an integrated approach to assessing the resistance of cotton to pathogens.
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Sobre autores
I. Akhmedzhanov
Institute of Biophysics and Biochemistry at the National University of Uzbekistan
Email: iskakhm@mail.ru
Tashkent, Uzbekistan
M. Khotamov
Institute of Genetics and Plant Experimental Biology of the Academy of Sciences of the Republic of Uzbekistan
Email: mansurhatamov@mail.ru
Yukori-Yuz, Uzbekistan
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