Formation of biologically active compounds by Siberian Cordyceps militaris (L.) Fr. strains

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Abstract

Fungal strains isolated in the Krasnoyarsk Territory from dead caterpillars of the Siberian silkworm Dendrolimus sibiricus Tschetverikov, found in the litter and crown of Abies sibirica Ledeb., based on morphological and genetic methods, were assigned to the species Cordyceps militaris (L.) Fr. The strains are highly active producers of bioactive compounds such as polysaccharides (PS), adenosine and cordysinin B. Cordysinin B was first discovered in the species C. militaris. It was found that the production of PS was more influenced by the method of growing mushrooms than by the carbon substrate. The highest production of PS (6.0‒6.7 g/l) and nucleosides (390 mg/l) of C. militaris 11-5 was observed during submerged cultivation on sucrose using aminopeptide. When studying the bio synthesis of PS during the growth of the fungus, it was found that the synthesized PS are consumed by the culture when the concentration of the carbon substrate in the environment decreases and, apparently, s erve as reserve sources of carbon.

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About the authors

T. V. Antipova

FRC Pushchino Centre for Biological Research, Russian Academy of Sciences; All-Russian Institute of Plant Protection

Author for correspondence.
Email: tatantip@rambler.ru

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms

Russian Federation, Pushchino, 142290; Pushkin, 196608, Saint-Petersburg

V. P. Zhelifonova

FRC Pushchino Centre for Biological Research, Russian Academy of Sciences

Email: tatantip@rambler.ru

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms

Russian Federation, Pushchino, 142290

B. P. Baskunov

FRC Pushchino Centre for Biological Research, Russian Academy of Sciences

Email: tatantip@rambler.ru

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms

Russian Federation, Pushchino, 142290

Y. A. Litovka

V.N. Sukachev Institute of Forest, FRC KSC, Siberian Branch, Russian Academy of Sciences; Reshetnev Siberian State University of Science and Technology

Email: tatantip@rambler.ru
Russian Federation, Krasnoyarsk, 660036; Krasnoyarsk, 660037

М. М. Patrusheva

V.N. Sukachev Institute of Forest, FRC KSC, Siberian Branch, Russian Academy of Sciences; Reshetnev Siberian State University of Science and Technology

Email: tatantip@rambler.ru
Russian Federation, Krasnoyarsk, 660036; Krasnoyarsk, 660037

V. B. Keshelava

Institute for Biological Instrumentation of the Russian Academy of Sciences

Email: tatantip@rambler.ru
Russian Federation, Pushchino, 142290

E. S. Mikhaylov

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; RosBioTech University at Pushchino

Email: tatantip@rambler.ru

Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Fundamental Biotechnology Department, RosBioTech University at Pushchino

Russian Federation, Pushchino, 142290; Pushchino, 142290

I. N. Chistiakov

ООО “Pushchino Biotechnologies”

Email: tatantip@rambler.ru
Russian Federation, Pushchino, 142290

I. N. Pavlov

V.N. Sukachev Institute of Forest, FRC KSC, Siberian Branch, Russian Academy of Sciences; Reshetnev Siberian State University of Science and Technology

Email: tatantip@rambler.ru
Russian Federation, Krasnoyarsk, 660036; Krasnoyarsk, 660037

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Supplementary files

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2. Fig. 1. Morphology of C. militaris 11-3 colonies: a ‒ fungal mycelium on the Dendrolimus sibiricus caterpillar; b ‒ colonies on MEA; c ‒ colonies on KSA (7 days); d ‒ colonies (left) and reverse colony (right) during culture aging on KSA (14 days).

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3. Fig. 2. Asexual reproduction of the C. militaris strain 11-3 on SNA medium (7 days).

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4. Fig. 3. Phylogenetic tree based on the maximum likelihood (ML) method for strain 11-3 (Siberia) and C. militaris sequences from NCBI GenBank: a ‒ using ITS primers (bootstrap values ​​greater than 40% are indicated next to the clusters); b ‒ using TEF-1α primers (bootstrap values ​​greater than 50% are indicated next to the clusters). Metarhizium rileyi was used as an outgroup.

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5. Fig. 4. Chromatogram of extracts under UV light (λ = 254 nm) from the biomass of C. militaris strains: 1 ‒ 11-3; 2 ‒ 11-4; 3 ‒ 11-5; 4 ‒ cordycepin standard.

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6. Fig. 5. Dynamics of growth, PS synthesis and carbohydrate consumption in C. militaris 11-5: 1 ‒ biomass, g/l; 2 – carbohydrates, g/l; 3 – PS, g/l.

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