Effect of environmental factors on recombinant activity of root nodule bacteria

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Abstract

The legume-rhizobia symbiosis is a unique natural phenomenon, which supplies the plant with the necessary mineral nitrogen via fixation of atmospheric dinitrogen. This interaction involves two partners: the legume plant and root nodule bacteria (rhizobia). In the wild, members of the Fabaceae family enter into symbiosis with a polymorphic group of rhizobia specific to them; the mechanism and reasons for the formation of heterogeneity of rhizobia are currently the subject of active research. In the present work, a Rhizobium leguminosarum strain strictly specific to Phaseolus vulgaris L. was used to show that within 30 days upon its introduction into soil, genetic rearrangements occurred in the cells, as was revealed by changes in the pattern of its genetic profile. It was also found that recombination activity of thecells was also affected by the root exudates produced during seed germination, which may indicate involvement of the plant in the formation of polymorphism of its microsymbionts. These findings suggest interpretation of this process not as a spontaneous event, but rather as the event controlled by the plant.

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About the authors

An. Kh. Baymiev

Ufa Research Center, Russian Academy of Sciences

Author for correspondence.
Email: baymiev@anrb.ru

Institute of Biochemistry and Genetics

Russian Federation, Ufa

I. S. Koryakov

Ufa Research Center, Russian Academy of Sciences

Email: baymiev@anrb.ru

Institute of Biochemistry and Genetics

Russian Federation, Ufa

Е. S. Akimova

Ufa Research Center, Russian Academy of Sciences

Email: baymiev@anrb.ru

Institute of Biochemistry and Genetics

Russian Federation, Ufa

А. А. Vladimirova

Ufa Research Center, Russian Academy of Sciences

Email: baymiev@anrb.ru

Institute of Biochemistry and Genetics

Russian Federation, Ufa

Al. Kh. Baymiev

Ufa Research Center, Russian Academy of Sciences

Email: baymiev@anrb.ru

Institute of Biochemistry and Genetics

Russian Federation, Ufa

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2. Fig. 1. Phoregram of RAPD profiles of rhizobia isolates obtained from common bean nodules using AFK-1 primer: A ‒ profiles of isolates obtained from nodules of plants grown in soil inoculated with R. leguminosarum strains Pvu2 and St4 after 30-day incubation; B ‒ profiles of isolates obtained from nodules of plants grown in sterile sand immediately after inoculation with R. leguminosarum strains Pvu2 and St4; B ‒ profile of strain Pvu2; G ‒ profile of strain St4; M ‒ 100 bp marker.

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3. Fig. 2. Phoregram of RAPD profiles of rhizobia isolates obtained from common bean nodules inoculated with bacteria after 30-day incubation with root secretions of seedlings using the AFK-1 primer. A – profile of the original strain Pvu2.

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4. Fig. 3. Foregram of RAPD profiles of rhizobia isolates isolated from common bean nodules using AFK-1 primer. A ‒ profiles of isolates obtained from nodules of a plant grown in soil inoculated with R. leguminosarum strain Pvu2 after 30-day incubation; B ‒ profiles of isolates obtained from nodules of a plant grown in the root system of common pea in soil inoculated with R. leguminosarum strain Pvu2; B ‒ profile of strain Pvu2; G ‒ profile of strain St4; M ‒ 100 bp marker.

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