Comparative Genome Analysis and Assessment of the Functional Properties of Streptococcus thermophilus Strains
- Authors: Moiseenko K.V.1, Glazunova O.A.1, Savinova O.S.1, Fedorova T.V.1
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Affiliations:
- Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences
- Issue: Vol 93, No 3 (2024)
- Pages: 340-345
- Section: SHORT COMMUNICATIONS
- URL: https://jdigitaldiagnostics.com/0026-3656/article/view/655105
- DOI: https://doi.org/10.31857/S0026365624030106
- ID: 655105
Cite item
Abstract
Streptococcus thermophilus are commonly used as starter cultures. Search for new safe strains with desired industrial and probiotic properties is an important issue. Complete genome sequencing was carried out, and the main genome characteristics of two new strains, S. thermophilus 16t (Str16t) and 159 (Str159), were determined. In silico analysis of the genomes revealed the absence of transmissible antibiotic resistance genes, virulence genes associated with pathogenicity, and integrated plasmids; gene clusters encoding class I and class II bacteriocin were found. In vitro tests showed phosphatase, peptidase, β-galactosidase, and esterase activity of both strains, as well as their ability to ferment glucose, lactose, sucrose, and ribose. Strain Str16t metabolized mannose as well. Str16t and Str159 are promising strains for application as starter and probiotic cultures.
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About the authors
K. V. Moiseenko
Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences
Email: fedorova_tv@mail.ru
Russian Federation, Moscow
O. A. Glazunova
Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences
Email: fedorova_tv@mail.ru
Russian Federation, Moscow
O. S. Savinova
Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences
Email: fedorova_tv@mail.ru
Russian Federation, Moscow
T. V. Fedorova
Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences
Author for correspondence.
Email: fedorova_tv@mail.ru
Russian Federation, Moscow
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