Effect of Staphylococcus aureus Cell-Free Culture Liquid on the Structure and Biochemical Composition of Klebsiella pneumoniae and Pseudomonas aeruginosa Biofilms

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Abstract

Recently acquired data suggest that many infections are associated with formation of multispecies biofilms, in which both antibiotic sensitivity and the permeability of the extracellular matrix differ from those of monocultures. In this work, we show that addition of cell-free culture liquid of Staphylococcus aureus to the biofilms of Klebsiella pneumoniae and Pseudomonas aeruginosa increased the content of α- and β-polysaccharides in the matrix up to twofold, which in turn probably affected the biofilm structure. Increased content of the polysaccharide component was also confirmed by a significantly increased expression of the K. pneumoniae pgaA gene and of the P. aeruginosa pelA and pslA genes in the presence of S. aureus culture liquid.

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About the authors

A. V. Mironova

Kazan (Volga Region) Federal University

Email: kairatr@yandex.ru
Russian Federation, Kazan

M. S. Fedorova

Kazan (Volga Region) Federal University

Email: kairatr@yandex.ru
Russian Federation, Kazan

N. D. Zakarova

Kazan (Volga Region) Federal University

Email: kairatr@yandex.ru
Russian Federation, Kazan

A. R. Salikhova

Kazan (Volga Region) Federal University

Email: kairatr@yandex.ru
Russian Federation, Kazan

E. Yu. Trizna

Kazan (Volga Region) Federal University

Email: kairatr@yandex.ru
Russian Federation, Kazan

A. R. Kayumov

Kazan (Volga Region) Federal University

Author for correspondence.
Email: kairatr@yandex.ru
Russian Federation, Kazan

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2. Fig. 1. Effect of cell-free culture fluid of S. aureus on the structure and matrix composition of K. pneumoniae (a, b, c) and P. aeruginosa (d, e, f) biofilms. Residual biofilm was assessed by crystal violet staining (a, d), biochemical composition (b, e), biofilm matrix distribution and structure (c, f) were evaluated using fluorescent dyes ConA-TMR, Calcofluor White M2R (CFW), Sypro Orange. Quantitative analysis of micrographs was performed using BioFilmAnalyzer software, the content of components as a percentage of control is indicated. Labelling corresponds to 50 µm

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3. Fig. 2. Relative expression of genes encoding extracellular polysaccharides of K. pneumoniae (a) and P. aeruginosa (b, c) biofilms without treatment (control) and in the presence of culture fluid of Staphylococcus aureus (+12% CJ)

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