Effect of O-Polysaccharide Modifications on Successful Plant Colonization by Bacteria

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Abstract

O-polysaccharides of gram-negative bacteria are a highly variable component of the lipopolysaccharide molecules located at the cell wall surface and involved in microbial interaction with plant and animal cells. Activity of prophage genes often results in various non-stoichiometric modifications (methylation, acetylation, etc.) of glycans at bacterial cell surface. The share of modified O-polysaccharides increases during the stationary growth phase and results in increased hydrophobicity of microbial surface. Bacterial cells with different hydrophobicity showed difference in attachment to plant roots. Increased cell hydrophobicity index was found to result in a significant increase in the number of adsorbed microorganisms per unit root length. Thus, acetyl transferase and methyl transferase genes of viral origin may be indirectly involved in successful colonization of plant roots by rhizosphere bacteria.

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About the authors

G. L. Burygin

Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences; Saratov State University; Saratov State University of Genetics, Biotechnology, and Engineering named after N.I. Vavilov

Author for correspondence.
Email: burygingl@gmail.com
Russian Federation, Saratov, 410049; Saratov, 410012; Saratov, 410012

A. A. Khanina

Saratov State University of Genetics, Biotechnology, and Engineering named after N.I. Vavilov

Email: burygingl@gmail.com
Russian Federation, Saratov, 410012

M. V. Filippova

Saratov State University of Genetics, Biotechnology, and Engineering named after N.I. Vavilov

Email: burygingl@gmail.com
Russian Federation, Saratov, 410012

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2. Fig. 1. Dependence of the adhesive activity of rhizosphere strains on potato roots on the index of hydrophobicity of bacterial cells. 1 — Ensifer adherens T1Ks14; 2 — Pseudomonas chlororaphis K3; 3 — Enterobacter ludwigii K7; 4 — Ochrobactrum cytisi IPA7.2; 5 — Azospirillum lipoferum SR65; 6 — Ochrobactrum quorumnocens T1Kr02; 7 — Azospirillum brasilense Sp7; 8 — Azospirillum lipoferum SR66; 9 — Azospirillum brasilense Jm6B2.

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