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Alkaline Phosphatase Activity and Phosphatase-Active Bacteria in Lake Baikal Water Column and Major Tributaries
- Authors: Suslova M.Y.1, Podlesnaya G.V.1, Tomberg I.V.1, Sakirko M.V.1, Belykh O.I.1
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Affiliations:
- Limnological Institute, Siberian Branch, Russian Academy of Sciences
- Issue: Vol 93, No 2 (2024)
- Pages: 223-227
- Section: SHORT COMMUNICATIONS
- URL: https://jdigitaldiagnostics.com/0026-3656/article/view/655140
- DOI: https://doi.org/10.31857/S0026365624020235
- ID: 655140
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Abstract
Phosphorus is one of the major biogenic elements. Its inflow facilitates eutrophication of lake water. In aquatic ecosystems, phosphorus is present mostly in organic compounds. Ability of aquatic microorganisms to assimilate phosphorus from organophosphorous compounds results from activity of alkaline phosphatases; activity of these enzymes may be an indicator of the state of the ecosystem, phosphate load, and water quality. In the present work, alkaline phosphatase activity (APA) and abundance of phosphatase-active bacteria (PAB) in Lake Baikal pelagic zone and in the mouths of its major tributaries was studied. In the pelagic zone, APA and PAB abundance decreased with depth, indicating that the main processes of phosphate generation occurred in the trophic layer of the lake. In the main tributaries, both APA and PAB abundance were considerably higher than in the pelagic zone. These results indicate active biochemical processes of transformation of organophosphorous compounds occur in the estuarine zones of the rivers. The degradation processes result in regeneration of phosphates, which are completely incorporated in the biological turnover, providing for phytoplankton development.
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About the authors
M. Yu. Suslova
Limnological Institute, Siberian Branch, Russian Academy of Sciences
Author for correspondence.
Email: suslova@lin.irk.ru
Russian Federation, Irkutsk, 664033
G. V. Podlesnaya
Limnological Institute, Siberian Branch, Russian Academy of Sciences
Email: suslova@lin.irk.ru
Russian Federation, Irkutsk, 664033
I. V. Tomberg
Limnological Institute, Siberian Branch, Russian Academy of Sciences
Email: suslova@lin.irk.ru
Russian Federation, Irkutsk, 664033
M. V. Sakirko
Limnological Institute, Siberian Branch, Russian Academy of Sciences
Email: suslova@lin.irk.ru
Russian Federation, Irkutsk, 664033
O. I. Belykh
Limnological Institute, Siberian Branch, Russian Academy of Sciences
Email: suslova@lin.irk.ru
Russian Federation, Irkutsk, 664033
References
- Максименко С.Ю., Парфенова В.В., Томберг И.В., Синюкович В.Н., Сороковикова Л.М., Поповская Г.И. Активность щелочной фосфатазы и динамика фосфора в дельте реки Селенга // Биология внутренних вод. 2007. № 4. С. 9–14
- Родина А.Г. Методы водной микробиологии. М.: Наука, 1968. 362 с.
- Сороковикова Л.М., Синюкович В.Н., Иванов В.Г., Елецкая Е.В., Моложникова Е.В., Башенхаева Н.В., Томберг И.В. Появление биогенных элементов с водами реки Баргузин и их роль в эвтрофировании Баргузинского залива (озеро Байкал) // Метеорология и гидрология. 2022. № 2. С. 75–86.
- Sorokovikova L.M., Sinyukovich V.N., Ivanov V.G., Eletskaya E.V., Molozhnikova E.V., Bashenkhaeva N.V., Tomberg I.V. Inflow of biogenic elements with the Barguzin River water and their role in eutrophication of the Barguzin Bay (Lake Baikal) // Russ. Meteorol. Hydrol. 2022. V. 47. P. 123–132.
- Сороковикова Л.М., Томберг И.В., Синюкович В.Н., Моложникова Е.В., Елецкая Е.В. Фосфор в воде р. Селенги и его вынос в оз. Байкал в условиях низкой водности // География и природные ресурсы. 2018. Т. 39. № 4. C. 88–94.
- Sorokovikova L.M., Tomberg I.V., Sinyukovich V.N., Molozhnikov E.V., Eletskaya E.V. Phosphorus in the Selenga River water and its input to Lake Baikal in conditions of low hydraulicity // Geogr. Nat. Resour. 2018. Т. 39. Р. 343–348.
- Хупер Ф. Происхождение и судьба органических соединений фосфора в водных системах // Фосфор в окружающей среде / Под ред. Федоровой М.М.: Мир, 1977. С. 204‒231.
- Belykh O.I., Sorokovikova E.G. Autotrophic picoplankton in Lake Baikal: Abundance, dynamics, and distribution // Aquat. Ecosyst. Health. Manage. 2003. V. 6. P. 251–261.
- Boavida M., Heath R. Is alkaline phosphatase always important in phosphate regeneration? // Arch. Hydrobiol. 1988. V. 111. P. 507‒518.
- Cabello-Yeves P.J., Callieri C., Picazo A., Schallenberg L., Huber P., Roda-Garcia J.J., Bartosiewicz M., Belykh O.I., Tikhonova I.V., Torcello-Requena A., De Prado P.M., Puxty R.J., Millard A.D., Camacho A., Rodriguez-Valera F., Scanlan D.J. Elucidating the picocyanobacteria salinity divide through ecogenomics of new freshwater isolates // BMC Biology. 2022. V. 20. P. 1–24.
- Chrost R.J., Overbeck J. Kinetics of alkaline phosphatase activity and phosphorus availability for phytoplankton and bacterioplankton in Lake Plubsee (North German eutrophic lake) // Microb. Ecol. 1987. V. 13. P. 229–248.
- Domysheva V., Vorobyeva S., Golobokova L., Netsvetaeva O., Onischuk N., Sakirko M., Khuriganova O., Fedotov A. Assessment of the current trophic status of the Southern Baikal littoral zone // Water. 2023. V. 15. Art. 1139.
- Eletskaya E.V., Tomberg I.V. The concentration of mineral and total phosphorus in the coastal water of southeast coast of Lake Baikal // Limnol. Freshwater Biol. 2020. № 4. P. 896–898.
- Labry C., Delmas D., Youenou A., Quere J., Leynaert A., Fraisse S., Raimonet M., Ragueneau O. High alkaline phosphatase activity in phosphate replete waters: the case of two macrotidal estuaries // Limnol. Oceanogr. 2016. V. 61. P. 1513–1529.
- Martinez J., Azam F. Periplasmic aminopeptidase and alkaline phosphatase activities in a marine bacterium: implications for substrate processing in the sea // Mar. Ecol. Prog. Ser. 1993. V. 92. P. 89–97.
- Miettinen I.T., Vartiainen T., Martikainen P.J. Phosphorus and bacterial growth in drinking water // Appl. Environ. Microbiol. 1997. V. 63. P. 3242‒3245.
- Suslova M.Yu., Sukhanova E.V., Shtykova Yu.R., Podlesnaya G.V., Galachyants A.D., Zimens E.A., Belykh O.I. Distribution and number of cultured bacteria of the phosphorus cycle in the ecosystem of Lake Baikal // Limnol. Freshwater Biol. 2020. № 4. P. 1037–1039.
- Wynne D., Bergstein Ben-Dan T. The effect of light and phosphate concentrations on phosphatase activities of the photosynthetic bacterium Chlorobium spp. // Can. J. Microbiol. 1995. V. 41. P. 278–283.
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