Genomic and functional analysis of a representative of the rare genus Krasilnikoviella ‒ Strain YrIIa5 with multiple antibiotic resistance

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Abstract

The increasing interest in the so-called “rare genera” of actinomycetes is associated with insufficiently studied of their potential as producers of valuable metabolites, primarily antibiotics. The use of selective methods made it possible to isolate the YrIIa5 strain from the soil collected near the “Dutch Fortress” (Rostov, Yaroslavl Region). Phylogenetic analysis based on the similarity of the 16S rRNA genes and five housekeeping genes (MLSA) allowed us to assign the isolate to the genus Krasilnikoviella. Genome analysis revealed many genes responsible for resistance to a wide range of antibiotics, which was confirmed by studying the strain for sensitivity to antimicrobial compounds using the agar diffusion method.

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About the authors

A. R. Kiriy

Sirius University of Science and Technology, Scientific Center for Translational Medicine

Email: metrim@gmail.com
Russian Federation, Sochi, 354340

Yu. V. Zakalyukina

Sirius University of Science and Technology, Scientific Center for Translational Medicine; Lomonosov Moscow State University

Email: metrim@gmail.com
Russian Federation, Sochi, 354340; Moscow, 119234

Yu. A. Buyuklyan

Sirius University of Science and Technology, Scientific Center for Translational Medicine

Email: metrim@gmail.com
Russian Federation, Sochi, 354340

M. V. Biryukov

Sirius University of Science and Technology, Scientific Center for Translational Medicine; Lomonosov Moscow State University; Center of Life Sciences, Skolkovo Institute of Science and Technology

Author for correspondence.
Email: metrim@gmail.com
Russian Federation, Sochi, 354340; Moscow, 119234; Moscow, 121205

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2. Fig. 1. Phylogram based on six concatenated sequences of housekeeping genes (16S rRNA, atpD, gyrB, recA, rpoB, trpB, 10751 bp in total), constructed by the maximum likelihood method with the highest log-likelihood (‒54397.04). The scale bar corresponds to 5 substitutions per 100 nucleotides. Clusters with a high level of confidence (bootstrap >70%), also confirmed by the Minimum Evolution, UPGMA, Neighbor-Joining methods in the MEGA v.11 program, are marked with an asterisk.

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3. Fig. 2. Electron micrographs of the strain Krasilnikoviella sp. YrIIa5 after 48 hours (a) and 6 days (b) of incubation on Organic 79 medium at 28°C.

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