Phytostimulation Activity of Methylobacterium dichloromethanicum subsp. dichloromethanicum DM4 and Its groEL2 Gene Knockout Mutant

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For the first time, the genome of the dichloromethane destructor Methylobacterium dichloromethanicum subsp. dichloromethanicum DM4 was analyzed for the presence of genetic determinants indicating its potential as a plant growth stimulator, and the ability of this strain and its groEL2 gene mutant for the to improve plant growth was determined. The genome of strain DM4 contains genes involved in the biosynthesis of phytohormones (indolyl-3-acetic acid and cytokinins), siderophores, carotenoids, poly-β-hydroxybutyrate, hydrolytic enzymes, as well as enzymes involved in the degradation of D-cysteine, protection from UV-damage and phosphate solubilization. Inoculation of lettuce sprouts by strain DM4 had a positive effect on plant growth and development, and increased adaptive defense and resistance to short-term temperature stress in plant growth experiments. Comparative analysis of the production of auxins, siderophores, hydrolytic enzymes, D-cysteine desulfohydrase activity, and the ability to solubilize insoluble phosphates in strains DM4 and DM4 ΔgroEL2 showed that disruption of the groEL2 gene led to a decrease in the synthesis of indole derivatives and phosphate solubilizing ability in the mutant strain. Assessment of the impact of inoculation of lettuce plants by these strains also demonstrated a decrease in the phytostimulation potential of DM4 ΔgroEL2 compared to the original strain. The data obtained indicate that the chaperonin GroEL2 in M. dichloromethanicum subsp. dichloromethanicum DM4 indirectly affects its phytostimulation activity.

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Sobre autores

N. Agafonova

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences

Autor responsável pela correspondência
Email: nadyagafonova@gmail.com
Rússia, Pushchino, 142290

G. Ekimova

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences

Email: nadyagafonova@gmail.com
Rússia, Pushchino, 142290

Y. Firsova

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences

Email: nadyagafonova@gmail.com
Rússia, Pushchino, 142290

M. Torgonskaya

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences

Email: nadyagafonova@gmail.com
Rússia, Pushchino, 142290

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2. Fig. 1. Distribution of categories of subsystems of cellular metabolism of M. dichloromethanicum subsp. dichloromethanicum DM4 based on the results of functional annotation according to the KEGG BlastKOALA (a) and RAST (b) databases.

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3. 2. Phosphorus accumulation in the culture fluid (a) and optical density of cells (b) during cultivation of M. dichloromethanicum subsp. dichloromethanicum DM4 (1) and DM4 ΔgroEL2 (2) on a mineral medium with Ca3(PO4)2 as the only source of phosphorus.

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4. 3. The activity of antioxidant protection enzymes catalase (a) and peroxidase (b), as well as the concentration of endogenous MDA (c) in lettuce plants inoculated with strains of M. dichloromethanicum subsp. dichloromethanicum DM4 and DM4 ΔgroEL2 and uninoculated (control), under normal conditions (1) and after exposure to temperature stress (2). Significant differences (p < 0.05) between the variants are marked with different letters.

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