Types of pesticides interaction in mixtures: results of inhibitory analysis

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Abstract

Enzymatic inhibitory assay based on the coupled enzyme system NAD(P)·H:FMN oxidoreductase and luciferase (Red + Luc), originally developed for environmental monitoring of soils, water and air, is proposed as a method for evaluating changes in the properties of active ingredients of pesticide preparations depending on the additional components (formulants), as well as when pesticides are combined in mixtures. Using the commercial pesticide preparations containing glyphosate it was shown that the degree of inhibition of the coupled enzyme system Red + Luc is depended on the formulants rather than the active ingredient in their composition. Moreover, the combined inhibitory effect of the pesticides in a mixture was not additive. According to the results of the inhibitory assay, the type of interaction of pesticide preparations in mixtures depends on both the formulants used and the ratio of pesticides in the mixture.

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About the authors

Е. N. Esimbekova

Siberian Federal University; Institute of Biophysics of Siberian Branch of Russian Academy of Sciences

Author for correspondence.
Email: esimbekova@yandex.ru
Russian Federation, Krasnoyarsk; Krasnoyarsk

D. V. Satir

Siberian Federal University

Email: esimbekova@yandex.ru
Russian Federation, Krasnoyarsk

V. А. Kratasyuk

Siberian Federal University; Institute of Biophysics of Siberian Branch of Russian Academy of Sciences

Email: esimbekova@yandex.ru
Russian Federation, Krasnoyarsk; Krasnoyarsk

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2. Fig. 1. Dependence of residual luminescence of the bienzyme system Red + Luc on the concentration of A) glyphosate in various commercial preparations: 1 – “Tornado”, 2 – “Glider”, 3 – “Liquidator”; B) the concentration of copper oxychloride in the commercial preparation “Abiga-Peak”. The data are presented as M ± m, n = 5.

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3. Fig. 2. Dependence of the residual luminescence of the Red + Luc bienzyme system on the ratio of glyphosate (in the composition of the “Liquidator” pesticide) and copper oxychloride (in the composition of the “Abiga-Peak” pesticide) in the reaction mixture.

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4. Fig. 3. Dependence of the residual luminescence of the Red + Luc bienzyme system on the ratio of glyphosate (in the Tornado pesticide) and copper oxychloride (in the Abiga-Peak pesticide) in the reaction mixture.

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Presented by Academician of the RAS A.G. Degemedzhi


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