Antitumor effect of helium ions with energy of 320 MeV/ion during irradiation of Ehrlich ascites carcinoma cells ex vivo

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Abstract

The regularities of tumor induction and growth were studied in mice exposed to a single ex vivo irradiation with a helium ion beam of Ehrlich adenocarcinoma ascites cells (EAC) at doses of 10 Gy and 20 Gy in two positions of the Bragg curve (before the peak and at the peak) in comparison with X-ray radiation at the same doses. It was shown that the frequency of induction and delay in tumor appearance depend on the dose of helium ion irradiation. The following were determined: the time of a fivefold increase in the EAC volume, tumor growth inhibition, tumor growth index (TGI), and increase in life expectancy (ILE) in mice. A decrease in the TGI values and an increase in the ILE values occurred with increasing dose for all types of radiation. The relative biological effectiveness value for helium ions determined by the area under the EAC growth dynamics curves reached a maximum value of 1.8 upon irradiation at the Bragg peak at a dose of 20 Gy.

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About the authors

V. E. Balakin

Lebedev Physical Institute, Russian Academy of Sciences

Email: strelnikova.ns@lebedev.ru

Corresponding Member of the RAS, Physical-Technical Center

Russian Federation, Protvino

N. S. Strelnikova

Lebedev Physical Institute, Russian Academy of Sciences

Author for correspondence.
Email: strelnikova.ns@lebedev.ru

Physical-Technical Center 

Russian Federation, Protvino

O. M. Rozanova

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: strelnikova.ns@lebedev.ru
Russian Federation, Pushchino

E. N. Smirnova

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: strelnikova.ns@lebedev.ru
Russian Federation, Pushchino

T. A. Belyakova

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences; Institute for High Energy Physics named by A.A. Logunov of National Research Centre “Kurchatov Institute”

Email: strelnikova.ns@lebedev.ru
Russian Federation, Pushchino; Protvino

A. E. Shemyakov

Lebedev Physical Institute, Russian Academy of Sciences; 2Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: strelnikova.ns@lebedev.ru

Physical-Technical Center 

Russian Federation, Protvino; Pushchino

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2. Fig. 1. Dynamics of solid EAC growth after implantation of irradiated EAC cells with 4He before the peak, at the Bragg peak and RI at doses of 10 Gy (a) and 20 Gy (b). Statistical significance was assessed using Student's t-test, where * – p≤0.05, ** – p≤0.01.

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3. Fig. 2. Dependence of the magnitude of tumor growth inhibition in mice on the time after implantation of EAC cells irradiated with 4He before and at the Bragg peak and RI at doses of 10 Gy (a) and 20 Gy (b).

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4. Fig. 3. Tumor growth rate in mice after ex vivo irradiation of tumor cells with 4He and RI at doses of 10 Gy and 20 Gy. Volumes are normalized to the first measured volume (>0.40 cm3). Statistical significance from the control group was assessed using the Mann-Whitney U-test (* – p≤0.05, ** – p≤0.01).

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