Cellulolytic potential of new strains of soil streptomycetes

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Abstract

In order to obtain new strains of cellulolytic microorganisms for use in technologies for processing plant and other cellulose-containing waste, four new bacterial strains of the genus Streptomyces have been isolated from various sources. In tests with Congo red, the isolates’ ability to degrade carboxymethylcellulose (CMC), microcrystalline cellulose (MCC) and natural cellulose–containing materials – straw, birch and oak sawdust was determined. The activity of cellulase was quantified during fermentation of straw. On media with each of the model carbon sources, the radial growth rate was determined as an indicator characterizing the ability of isolates to colonize various substrates. The conjugate characteristic of cellulase activity and radial growth rate on substrates of various natures made it possible to assess the prospects for further use of the studied strains as destructors of cellulose-containing waste. The strain Streptomyces thermocarboxydus T1-3 was the leader in the studied sample, characterized by the most active enzymatic hydrolysis of all model cellulose sources, a high radial growth rate (up to 87±3 microns/hour), and the achievement of maximum cellulase activity (171.25±8.13 U/ml) during straw fermentation within 24 hours.

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About the authors

N. A. Bokov

Federal Agricultural Scientific Center of the North-East named N.V.Rudnitsky; Vyatka State University

Author for correspondence.
Email: nikita-bokov@mail.ru
Russian Federation, 610007, Kirov; 610000, Kirov

A. V. Bakulina

Federal Agricultural Scientific Center of the North-East named N.V.Rudnitsky

Email: nikita-bokov@mail.ru
Russian Federation, 610007, Kirov

E. A. Bessolitsyna

Federal Agricultural Scientific Center of the North-East named N.V.Rudnitsky

Email: nikita-bokov@mail.ru
Russian Federation, 610007, Kirov

I. G. Shirokikh

Federal Agricultural Scientific Center of the North-East named N.V.Rudnitsky; Vyatka State University

Email: nikita-bokov@mail.ru
Russian Federation, 610007, Kirov; 610000, Kirov

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2. Fig. 1. Heat map reflecting the differences in the size of the zones of hydrolysis of cellulose by streptomycetes in the composition of various substrates: 1 – oak sawdust; 2 – straw; 3 – birch sawdust; 4 – CMC; 5 – MCC.

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3. Fig. 2. Dynamics of the radial growth rate on media with different carbon sources of strains: 1 – S. griseoaurantiacus Мb4-2; 2 – S. thermocarboxydus T1-3; 3 – S. hygroscopicus N27-25; 4 – “S. ryensis” Н13-3.

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4. Fig. 3. The rate of radial growth on different substrates of strains: 1 – S. griseoaurantiacus Мb4-2; 2 – S. thermocarboxydus T1-3; 3 – S. hygroscopicus N27-25; 4 – “S. ryensis” Н13-3 (average for 14 days).

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