Search for Bacteriophages Specific against Members of the Genus Rhodococcus

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

This is the first report on the isolation of Rhodococcus aetherivorans-specific bacteriophages and of applicability of such invertebrates as Hyalophora cecropia and Eisenia fetida as objects for screening the phage microflora of Rhodococcus species. Some of the isolated phages were capable of growth of R. ruber and R. qingshengii. An efficient procedure for bacteriophage reproduction in the liquid culture of R. aetherivorans was developed. The revealed bacteriophages may be used for development of efficient genetic tools for Rhodococcus strains, including the industrially significant ones.

Full Text

Restricted Access

About the authors

A. D. Novikov

NRC “Kurchatov Institute”, Kurchatov Genomic Center

Author for correspondence.
Email: alexm19@mail.ru
Russian Federation, 123182

I. P. Tokmakova

NRC “Kurchatov Institute”, Kurchatov Genomic Center

Email: alexm19@mail.ru
Russian Federation, 123182

A. A. Samarin

NRC “Kurchatov Institute”, Kurchatov Genomic Center

Email: alexm19@mail.ru
Russian Federation, 123182

K. V. Lavrov

NRC “Kurchatov Institute”, Kurchatov Genomic Center

Email: alexm19@mail.ru
Russian Federation, 123182

A. S. Yanenko

NRC “Kurchatov Institute”, Kurchatov Genomic Center

Email: alexm19@mail.ru
Russian Federation, 123182

References

  1. Бактериофаги. Биология и практическое применение / Под ред. Э. Каттер, А. М. Сулаквелидзе. М.: Научный мир, 2012. 640 с.
  2. Bubnov D. M., Yuzbashev T. V., Khozov A. A., Melkina O. E., Vybornaya T. V., Stan G. B., Sineoky S. P. Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs // Nucleic Acids Res. 2022. V. 50. P. 8947–8960. https://doi.org/10.1093/nar/gkac649
  3. Grechishnikova E. G., Shemyakina A. O., Novikov A. D., Lavrov K. V., Yanenko A. S. Rhodococcus: sequences of genetic parts, analysis of their functionality, and development prospects as a molecular biology platform // Crit. Rev. Biotechnol. 2023. V. 43. P. 835–850. https://doi.org/10.1080/07388551.2022.2091976
  4. Guzman J., Vilcinskas A. Draft genome sequence of Rhodococcus rhodochrous strain G38GP, isolated from the Madagascar hissing cockroach // Microbiol. Resour. Announc. 2021. V. 10. Art. e0077721. https://doi.org/10.1128/MRA.00777-21
  5. Kim D., Choi K. Y., Yoo M., Zylstra G. J., Kim E. Biotechnological potential of Rhodococcus biodegradative pathways // J. Microbiol. Biotechnol. 2018. V. 28. P. 1037–1051. https://doi.org/10.4014/jmb.1712.12017
  6. Larkin M. J., Kulakov L. A., Allen C. C. Biodegradation and Rhodococcus – masters of catabolic versatility // Curr. Opin. Biotechnol. 2005. V. 16. P. 282–290. https://doi.org/10.1016/j.copbio.2005.04.007
  7. Liang Y., Jiao S., Wang M., Yu H., Shen Z. A CRISPR/Cas9-based genome editing system for Rhodococcus ruber TH // Metab. Engin. 2020. V. 57. P. 13–22. https://doi.org/10.1016/j.ymben.2019.10.003
  8. Liang Y. X., Yu H. M. Genetic toolkits for engineering Rhodococcus species with versatile applications // Biotechnol. Adv. 2021. V. 49. Art. 107748. https://doi.org/10.1016/j.biotechadv.2021.107748
  9. Martinkova L., Uhnakova B., Patek M., Nesvera J., Kren V. Biodegradation potential of the genus Rhodococcus // Environ. Int. 2009. V. 35. P. 162–177. https://doi.org/10.1016/j.envint.2008.07.018
  10. Salcedo-Porras N., Umana-Diaz C., de Oliveira Barbosa Bitencourt R., Lowenberger C. The role of bacterial symbionts in triatomines: an evolutionary perspective // Microorganisms. 2020. V. 8. Art. 1438. https://doi.org/10.3390/microorganisms8091438
  11. Summer E. J., Liu M., Gill J. J., Grant M., Chan-Cortes T.N., Ferguson L., Janes C., Lange K., Bertoli M., Moore C., Orchard R. C., Cohen N. D., Young R. Genomic and functional analyses of Rhodococcus equi phages ReqiPepy6, ReqiPoco6, ReqiPine5, and ReqiDocB7 // Appl. Environ. Microbiol. 2011. V. 77. P. 669–683. https://doi.org/10.1128/AEM.01952-10
  12. Yassin A. F. Rhodococcus triatomae sp. nov., isolated from a blood-sucking bug // Int. J. Syst. Evol. Microbiol. 2005. V. 55. P. 1575–1579. https://doi.org/10.1099/ijs.0.63571-0
  13. Патент СССР. 1990. № SU1731814.
  14. Патент США. 2014. № US20140187818A1.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2024 Russian Academy of Sciences