Hydrocarbon-Oxidizing Bacteria of the Bottom Ecotopes of the Barents and Pechora Seas

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Abstract

Microorganisms capable of degrading hydrocarbons are regular components of natural microbial communities and play an important role in self-purification of marine environments from oil contamination. High-throughput sequencing of the 16S rRNA gene V4 variable region was used to analyze microbial communities of the Barents and Pechora seas and of the microcosms with a spectrum of hydrocarbon substrates: oil, n-nonane, n-undecane, and phenanthrene. The Barents Sea communities of hydrocarbon-oxidizing microorganisms were characterized by predominance of the genera Pseudoalteromonas, Pseudomonas, Porticoccus, and Oleispira, while those of the Pechora Sea contained members of the genera Rhodococcus, Dietzia, Sphingorhabdus, and Hyphomonas. Pure cultures of these microorganisms were shown to utilize the major oil hydrocarbons: n-alkanes, cycloalkanes, and aromatic compounds.

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About the authors

V. O. Pyrkin

Lomonosov Moscow State University

Author for correspondence.
Email: vladisluw@yandex.ru
Russian Federation, Moscow

L. A. Gavirova

Lomonosov Moscow State University

Email: vladisluw@yandex.ru
Russian Federation, Moscow

A. R. Stroeva

Lomonosov Moscow State University

Email: vladisluw@yandex.ru
Russian Federation, Moscow

A. Yu. Merkel

Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology, Russian Academy of Sciences

Email: vladisluw@yandex.ru
Russian Federation, Moscow

O. N. Vidishcheva

Lomonosov Moscow State University

Email: vladisluw@yandex.ru
Russian Federation, Moscow

A. G. Kalmykov

Lomonosov Moscow State University

Email: vladisluw@yandex.ru
Russian Federation, Moscow

E. A. Bonch-Osmolovskaya

Lomonosov Moscow State University; Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology, Russian Academy of Sciences

Email: vladisluw@yandex.ru
Russian Federation, Moscow; Moscow

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Supplementary files

Supplementary Files
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2. Fig. 1. Averaged phylogenetic composition of microcosm communities from bottom water and soil of the Barents (a) and Pechora (b) Seas obtained by NGS profiling on 16S rRNA gene

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3. Fig. 2. Histogram of ratios of biodegradation markers of n-alkanes (a) and aromatic compounds (b) by the studied pure cultures of microorganisms

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