Genetic Identification of Microsymbionts of the Legume Hedysarum arcticum B. Fedtsch, Growing on Samoylovsky Island in the Lena River Delta (Arctic Zone of Yakutia)

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

Bacterial strains isolated from root nodules of the legume plant Hedysarum arcticum B. Fedtsch growing on Samoylovsky Island in the Lena River delta (Arctic zone of Yakutia) were assigned to the genera Rhizobium (family Rhizobiaceae) and Mesorhizobium (Phyllobacteriaceae) of the order Hyphomicrobiales (class Alphaproteobacteria) according to the rrs gene sequencing data. According to phylogenetic analysis of concatemers of the atpD, dnaK, gyrB, and rpoB genes, the strains belonged to the species Rhizobium giardinii and Mesorhizobium norvegicum. The strains were shown to be facultative psychrotrophs growing at 5 and 28ºC. These microsymbionts are promising for further study of their symbiotic efficiency regarding other forage legume species, with an aim to establish highly productive agrophytocenoses in the Far North.

Full Text

Restricted Access

About the authors

D. S. Karlov

All-Russia Research Institute for Agricultural Microbiology

Author for correspondence.
Email: ds.karlov@arriam.ru
Russian Federation, Saint Petersburg

P. V. Guro

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
Russian Federation, Saint Petersburg

I. G. Kuznetsova

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
Russian Federation, Saint Petersburg

А. L. Sazanova

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
Russian Federation, Saint Petersburg

I. A. Alekhina

Arctic and Antarctic Research Institute

Email: ds.karlov@arriam.ru
Russian Federation, Saint Petersburg

N. Yu. Tikhomirova

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
Russian Federation, Saint Petersburg

N. N. Lashchinsky

Central Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences

Email: ds.karlov@arriam.ru
Russian Federation, Novosibirsk

А. А. Belimov

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
Russian Federation, Saint Petersburg

V. I. Safronova

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
Russian Federation, Saint Petersburg

References

  1. Котелина Н. С., Арчегова И. Б., Романов Г. Г., Турубанова Л. П. Особенности природопользования и перспективы природовосстановления на Крайнем Севере России. Екатеринбург: УрО РАН, 1998. 148 с.
  2. Стратегия развития Арктической зоны Российской Федерации и обеспечения национальной безопасности на период до 2035 года. Утверждена Указом Президента РФ № 645 от 26 октября 2020 г. URL: http://kremlin.ru/acts/news/64274
  3. Экологические основы управления продуктивностью агрофитоценозов восточноевропейской тундры / Под ред. Арчегова И. Б., Котелина Н. С., Грунина Л.К и др. Л.: Наука, 1991. 152 с.
  4. Amarger N., Macheret V., Laguerre G. Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris nodules // Int. J. Syst. Bacteriol. 1997. V. 47. P. 996–1006. https://doi.org/10.1099/00207713-47-4-996
  5. Andrews M., Andrews M. E. Specificity in legume-rhizobia symbiosis // Int. J. Mol. Sci. 2017. V. 18. Art. 705. https://doi.org/10.3390/ijms18040705
  6. Helene L. C. F., Dall’Agnol R. F., Delamuta J. R. M., Hungria M. Mesorhizobium atlanticum sp. nov., a new nitrogen-fixing species from soils of the Brazilian Atlantic Forest biome // Int. J. Syst. Evol. Microbiol. 2019. V. 69. P. 1800–1806. https://doi.org/10.1099/ijsem.0.003397
  7. Jarvis B. D.W., Pankhurst C. E., Patel J. J. Rhizobium loti, a new species of legume root nodule bacteria // Int. J. Syst. Evol. Microbiol. 1982. V. 32. P. 378–380. https://doi.org/10.1099/00207713-32-3-378
  8. Kabdullayeva T., Crosbie D. B., Marín M. Mesorhizobium norvegicum sp. nov., a rhizobium isolated from a Lotus corniculatus root nodule in Norway // Int. J. Syst. Evol. Microbiol. 2020. V. 70. P. 388–396. https://doi.org/10.1099/ijsem.0.003769
  9. Kumar S., Stecher G., Li M., Knyaz C., Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms // Mol. Biol. Evol. 2018. V. 35. P. 1547–1549. https://doi.org/10.1093/molbev/msy096
  10. Martens M., Delaere M., Coopman R., De Vos P., Gillis M., Willems A. Multilocus sequence analysis of Ensifer and related taxa // Int. J. Syst. Evol. Microbiol. 2007. V. 57. P. 489–503. https://doi.org/10.1099/ijs.0.64344-0
  11. Novikova N., Safronova V. Transconjugants of Agrobacterium radiobacter harbouring sym genes of Rhizobium galegae can form an effective symbiosis with Medicago sativa // FEMS Microbiol. Lett. 1992. V. 93. P. 261–268. https://doi.org/10.1111/j.1574-6968.1992.tb05107.x
  12. Safronova V. I., Kuznetsova I. G., Sazanova A. L. et al. Microvirga ossetica sp. nov., a species of rhizobia isolated from root nodules of the legume species Vicia alpestris Steven // Int. J. Syst. Evol. Microbiol. 2017. V. 67. P. 94–100. https://doi.org/10.1099/ijsem.0.001577
  13. Weir B. S., Turner S. J., Silvester W. D., Park D.-C., Young J. M. Mesorhizobium strains and Rhizobium leguminosarum nodulate native legume genera of New Zealand, while introduced legume weeds asre nodulated by Bradyrhizobium species // Appl. Environ. Microbiol. 2004. V. 70. P. 5980–5987. https://doi.org/10.1128/AEM.70.10.5980-5987.2004
  14. Zhao C. T., Wang E. T., Chen W. F., Chen W. X. Diverse genomic species and evidences of symbiotic gene lateral transfer detected among the rhizobia associated with Astragalus species grown in the temperate regions of China // FEMS Microbiol. Lett. 2008. V. 286. P. 263–273. https://doi.org/10.1111/j.1574-6968.2008.01282.x

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig. 1. Phylogenetic tree of the Rhizobium genus using 16S rRNA gene sequences. Figures (%) in the branching nodes - reliability by bootstrap analysis of 500 alternative trees

Download (206KB)
Indexing metadata
3. Fig. 2. Phylogenetic tree of Rhizobium representatives based on concatemers of rpoB, atpD and dnaK genes. Figures (%) in the branching nodes - reliability by bootstrap analysis of 500 alternative trees

Download (141KB)
Indexing metadata
4. Fig. 3. Phylogenetic tree of representatives of the genus Mesorhizobium constructed using 16S rRNA gene sequences. Figures (%) in the branching nodes - reliability by bootstrap analysis of 500 alternative trees

Download (220KB)
Indexing metadata
5. Fig. 4. Phylogenetic tree of Mesorhizobium genus representatives based on concatemers of rpoB, gyrB, atpD and dnaK genes. Figures (%) in branching nodes - reliability by bootstrap analysis of 500 alternative trees

Download (140KB)
Indexing metadata

Copyright (c) 2024 Russian Academy of Sciences