Genetic Identification of Microsymbionts of the Legume Hedysarum arcticum B. Fedtsch, Growing on Samoylovsky Island in the Lena River Delta (Arctic Zone of Yakutia)

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Bacterial strains isolated from root nodules of the legume plant Hedysarum arcticum B. Fedtsch growing on Samoylovsky Island in the Lena River delta (Arctic zone of Yakutia) were assigned to the genera Rhizobium (family Rhizobiaceae) and Mesorhizobium (Phyllobacteriaceae) of the order Hyphomicrobiales (class Alphaproteobacteria) according to the rrs gene sequencing data. According to phylogenetic analysis of concatemers of the atpD, dnaK, gyrB, and rpoB genes, the strains belonged to the species Rhizobium giardinii and Mesorhizobium norvegicum. The strains were shown to be facultative psychrotrophs growing at 5 and 28ºC. These microsymbionts are promising for further study of their symbiotic efficiency regarding other forage legume species, with an aim to establish highly productive agrophytocenoses in the Far North.

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D. Karlov

All-Russia Research Institute for Agricultural Microbiology

编辑信件的主要联系方式.
Email: ds.karlov@arriam.ru
俄罗斯联邦, Saint Petersburg

P. Guro

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
俄罗斯联邦, Saint Petersburg

I. Kuznetsova

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
俄罗斯联邦, Saint Petersburg

А. Sazanova

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
俄罗斯联邦, Saint Petersburg

I. Alekhina

Arctic and Antarctic Research Institute

Email: ds.karlov@arriam.ru
俄罗斯联邦, Saint Petersburg

N. Tikhomirova

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
俄罗斯联邦, Saint Petersburg

N. Lashchinsky

Central Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences

Email: ds.karlov@arriam.ru
俄罗斯联邦, Novosibirsk

А. Belimov

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
俄罗斯联邦, Saint Petersburg

V. Safronova

All-Russia Research Institute for Agricultural Microbiology

Email: ds.karlov@arriam.ru
俄罗斯联邦, Saint Petersburg

参考

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2. Fig. 1. Phylogenetic tree of the Rhizobium genus using 16S rRNA gene sequences. Figures (%) in the branching nodes - reliability by bootstrap analysis of 500 alternative trees

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3. Fig. 2. Phylogenetic tree of Rhizobium representatives based on concatemers of rpoB, atpD and dnaK genes. Figures (%) in the branching nodes - reliability by bootstrap analysis of 500 alternative trees

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4. Fig. 3. Phylogenetic tree of representatives of the genus Mesorhizobium constructed using 16S rRNA gene sequences. Figures (%) in the branching nodes - reliability by bootstrap analysis of 500 alternative trees

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5. Fig. 4. Phylogenetic tree of Mesorhizobium genus representatives based on concatemers of rpoB, gyrB, atpD and dnaK genes. Figures (%) in branching nodes - reliability by bootstrap analysis of 500 alternative trees

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