Inactivation of the gene responsible for the synthesis of gluconic acid in the genome of Pantoea brenneri

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Abstract. To study the contribution of the glucose dehydrogenase (gcd) gene product to the development of ISR priming in plants, a strain of Pantoea brenneri 3.2 with a deletion of the gcd gene was obtained. Using the Lambda Red (λ Red) phage recombination system, we obtained a marker-free mutant strain of P. brenneri 3.2 Δgcd. Inactivation of the gcd glucose dehydrogenase gene resulted in a 2.5-fold decrease in the strain’s ability to solubilize tricalcium phosphate on the solid nutrient medium NBRIP.

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Sobre autores

A. Suleimanova

Kazan (Volga Region) Federal University

Autor responsável pela correspondência
Email: Aliya.kzn@gmail.com
Rússia, Kazan, 420008

L. Sokolnikova

Kazan (Volga Region) Federal University

Email: Aliya.kzn@gmail.com
Rússia, Kazan, 420008

D. Bulmakova

Kazan (Volga Region) Federal University

Email: Aliya.kzn@gmail.com
Rússia, Kazan, 420008

E. Egorova

Kazan (Volga Region) Federal University

Email: Aliya.kzn@gmail.com
Rússia, Kazan, 420008

M. Sharipova

Kazan (Volga Region) Federal University

Email: Aliya.kzn@gmail.com
Rússia, Kazan, 420008

Bibliografia

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  12. Suleimanova A., Bulmakova D., Sokolnikova L., Egorova E., Itkina D., Kuzminova O., Gizatullina A., Sharipova M. Phosphate solubilization and plant growth promotion by Pantoea brenneri soil isolates // Microorganisms. 2023. V. 11. Art. 1136. https://doi.org/10.3390/microorganisms11051136

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2. Fig. 1. Electrophoresis of PCR products at the stages of obtaining the mutant strain P. brenneri 3.2 Δgcd

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3. Fig. 2. a – Growth of the native strain P. brenneri 3.2 and its mutant for the gene involved in the synthesis of gluconic acid on LB medium. *P < 0.05, **P < 0.01, ***P < 0.001; b – ability of the native and mutant strains to solubilize tricalcium phosphate.

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