A method for increasing the efficiency of selection of aptamers to cellular receptors
- 作者: Kuznetsova V.Е.1, Lebedev T.D.1, Shershov V.Е.1, Shtylev G.F.1, Shishkin I.Y.1, Miftahov R.A.1, Butvilovskaya V.I.1, Grechishnikova I.V.1, Zasedateleva О.А.1, Chudinov А.V.1
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隶属关系:
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- 期: 卷 51, 编号 3 (2025)
- 页面: 451-460
- 栏目: ОБЗОРНАЯ СТАТЬЯ
- URL: https://jdigitaldiagnostics.com/0132-3423/article/view/686963
- DOI: https://doi.org/10.31857/S0132342325030081
- EDN: https://elibrary.ru/KQMRJH
- ID: 686963
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A method has been proposed to increase the efficiency of selection of aptamers to cellular receptors by the cell-Selex method, in particular to the receptor tyrosine kinase c-KIT. The use of Tween 20 in buffer solutions in concentrations not exceeding 0.01%, as well as trypsinolysis of surface proteins at the stage of elution of the combinatorial library of oligonucleotides bound to the cell surface, led to an increase in the specificity of aptamers and a decrease in nonspecific sorption according to the results of fluorescence microscopy, thermofluorimetric analysis and high-precision sequencing.
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作者简介
V. Kuznetsova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: kuzneimb@gmail.com
俄罗斯联邦, ul. Vavilova 32, Moscow, 119991
T. Lebedev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
俄罗斯联邦, ul. Vavilova 32, Moscow, 119991
V. Shershov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
俄罗斯联邦, ul. Vavilova 32, Moscow, 119991
G. Shtylev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
俄罗斯联邦, ul. Vavilova 32, Moscow, 119991
I. Shishkin
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
俄罗斯联邦, ul. Vavilova 32, Moscow, 119991
R. Miftahov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
俄罗斯联邦, ul. Vavilova 32, Moscow, 119991
V. Butvilovskaya
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
俄罗斯联邦, ul. Vavilova 32, Moscow, 119991
I. Grechishnikova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
俄罗斯联邦, ul. Vavilova 32, Moscow, 119991
О. Zasedateleva
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
俄罗斯联邦, ul. Vavilova 32, Moscow, 119991
А. Chudinov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
俄罗斯联邦, ul. Vavilova 32, Moscow, 119991
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